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Update docs with info on specifying multiple chains in CdrBlast
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mikessh committed Jul 13, 2015
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21 changes: 11 additions & 10 deletions doc/cdrblast.rst
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Expand Up @@ -56,15 +56,15 @@ A sample metadata file could also be provided with
extraction. This file should have the following tab-separated table
structure:

+-------------+-----------+--------+----------------------+--------+--------------------------+
| Sample ID | Species | Gene | File types | Mask | Quality threshold pair |
+=============+===========+========+======================+========+==========================+
| S0 | human | TRA | paired, overlapped | 1:0 | 25,30 |
+-------------+-----------+--------+----------------------+--------+--------------------------+
| S1 | human | TRB | unpaired | - | 25,30 |
+-------------+-----------+--------+----------------------+--------+--------------------------+
| S2 | mouse | TRB | paired | 0:1 | 20,25 |
+-------------+-----------+--------+----------------------+--------+--------------------------+
+-------------+-----------+---------+----------------------+--------+--------------------------+
| Sample ID | Species | Gene | File types | Mask | Quality threshold pair |
+=============+===========+=========+======================+========+==========================+
| S0 | human | TRA | paired, overlapped | 1:0 | 25,30 |
+-------------+-----------+---------+----------------------+--------+--------------------------+
| S1 | human | TRB | unpaired | - | 25,30 |
+-------------+-----------+---------+----------------------+--------+--------------------------+
| S2 | mouse | TRB,TRA | paired | 0:1 | 20,25 |
+-------------+-----------+--------=+----------------------+--------+--------------------------+

See section below for more details.

Expand Down Expand Up @@ -107,7 +107,8 @@ to concatenate and process two or more FASTQ files at once:
java -jar migec.jar CdrBlast -R TRA checkout/S1_R2.fastq.gz checkout/S2_R2.fastq.gz cdrblast/S12_raw.cdrblast.txt
Gene parameter ``-R`` is required, supported genes are *TRA*, *TRB*,
*TRG*, *TRD*, *IGH*, *IGK* and *IGL*. Species could be provided with
*TRG*, *TRD*, *IGH*, *IGK* and *IGL*. Several chains can be specified,
for example ``-R TRA,TRB`` or ``-R IGH,IGL``. Species could be provided with
``-S`` parameter, by default uses *HomoSapiens*, supported species are
*HomoSapiens*, *MusMusculus* and others. Assembled data should be passed
to the script with ``-a`` option. ``--same-sample`` option should be
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