add new test script Prefill_plates_LysisBuffer

#9

EvoScriPy/labware.py

@@ -476,7 +476,7 @@ def get_labware(self, label: (str, int) = None, labw_type=None):
  if label in series.labels:
  return series.labels[label]
  raise Exception("ERROR: no labware '" + labw_type + "' with the label '" + label
- + "' was found in worktable: " + self.template_file_name)
+ + "' was found in worktable: " + str(self.template_file_name))
 
  if label is None:
  label = 0

protocols/Prefill_plates_LysisBuffer/Prefill_plates_LysisBuffer.ewt

@@ -38,8 +38,8 @@ V;200
 998;8;Washstation 2Grid Cleaner short;Washstation 2Grid Waste;Washstation 2Grid Cleaner long;Trough 100ml;Trough 100ml;Trough 100ml;Washstation 2Grid DiTi Waste;;
 998;;;;3-VEB Binding Buffer;2-Vl Lysis Buffer;7-EtOH80p;;;
 998;0;
-998;3;96 Well DeepWell square;;96 Well DeepWell square;
-998;Plate lysis;;Plate EtOH;
+998;3;96 Well DeepWell square;96 Well DeepWell square;96 Well DeepWell square;
+998;Plate lysis-1;Plate lysis-2;Plate lysis-3;
 998;0;
 998;0;
 998;0;

protocols/Prefill_plates_LysisBuffer/Prefill_plates_LysisBuffer.py

@@ -4,7 +4,7 @@
 # author Ariel Vina-Rodriguez (qPCR4vir)
 # 2018-2019
 __author__ = 'Ariel'
-# todo: deprecate, this is now one version in Prefill_plates_LysisBuffer_and_ProtKpreMix
+# this is now one version in Prefill_plates_LysisBuffer_and_ProtKpreMix
 
 from EvoScriPy.protocol_steps import *
 from protocols.evo100_f.evo100_f import Evo100_FLI
@@ -23,24 +23,19 @@ class Prefill_plates_LysisBuffer(Evo100_FLI):
  def def_versions(self):
  self.versions = {'3 plate': self.V_3_plate,
  '2 plate': self.V_2_plate,
- '1 plate': self.V_1_plate }
+ '1 plate': self.V_1_plate}
 
- def V_1_plate(self):
- self.num_plates = 1
-
- def V_2_plate(self):
- self.num_plates = 2
-
- def V_3_plate(self):
- self.num_plates = 3
+ def V_1_plate(self): self.num_plates = 1
+ def V_2_plate(self): self.num_plates = 2
+ def V_3_plate(self): self.num_plates = 3
 
  def __init__(self, GUI=None, run_name="Prefill plates with LysisBuffer"):
 
  this = Path(__file__).parent
 
  Evo100_FLI.__init__(self,
  GUI = GUI,
- num_of_samples= Prefill_plates_LysisBuffer.max_s,
+ num_of_samples = Prefill_plates_LysisBuffer.max_s,
  worktable_template_filename=this / 'Prefill_plates_LysisBuffer.ewt',
  output_filename = this / 'scripts' / 'Prefill_LysisBuffer',
  run_name = run_name)
@@ -54,12 +49,12 @@ def Run(self):
  self.comment('Prefill {:d} plates with LysisBufferReact for {:d} samples.'
  .format(self.num_plates, num_of_samples))
 
- # Get Labwares (Cuvette, eppys, etc.) from the work table
- LysBufCuvette = wt.get_labware("2-Vl Lysis Buffer", lab.Trough_100ml)
+  # Get Labwares (Cuvette, eppys, etc.) from the work table
+ LysBufCuvette = wt.get_labware("2-Vl Lysis Buffer", labware.Trough_100ml)
 
- DiTi1000_1 = wt.get_labware("1000-1", lab.DiTi_1000ul)
- DiTi1000_2 = wt.get_labware("1000-2", lab.DiTi_1000ul)
- DiTi1000_3 = wt.get_labware("1000-3", lab.DiTi_1000ul)
+ DiTi1000_1 = wt.get_labware("1000-1", labware.DiTi_1000ul)
+ DiTi1000_2 = wt.get_labware("1000-2", labware.DiTi_1000ul)
+ DiTi1000_3 = wt.get_labware("1000-3", labware.DiTi_1000ul)
 
 
  self.go_first_pos() # Set the initial position of the tips
@@ -68,16 +63,14 @@ def Run(self):
  LysisBufferVolume = 100.0 # VL1 or VL
 
  all_samples = range(num_of_samples)
- maxTips = min (self.n_tips, num_of_samples)
- maxMask = Rbt.tipsMask[maxTips]
 
  # Define the reactives in each labware (Cuvette, eppys, etc.)
 
  LysisBufferReact = Reagent("VL - Lysis Buffer ",
-  LysBufCuvette,
-  volpersample = LysisBufferVolume,
-  defLiqClass = 'MN VL',
-  num_of_samples= self.num_plates * num_of_samples)
+ LysBufCuvette,
+ volpersample = LysisBufferVolume,
+ defLiqClass = 'MN VL',
+ num_of_samples= self.num_plates * num_of_samples)
 
  # Show the check_list GUI to the user for possible small changes
 
@@ -86,7 +79,7 @@ def Run(self):
 
  instructions.wash_tips(wasteVol=5, FastWash=True).exec()
 
- LysPlat = [wt.get_labware("Plate lysis-" + str(i + 1), lab.MP96deepwell) for i in range(self.num_plates)]
+ LysPlat = [wt.get_labware("Plate lysis-" + str(i + 1), labware.MP96deepwell) for i in range(self.num_plates)]
 
  par = LysPlat[0].parallelOrder(self.n_tips, all_samples)
 

protocols/Prefill_plates_LysisBuffer/scripts/Prefill_LysisBuffer_1_plate.esc

@@ -0,0 +1,163 @@
+27325A93
+20190410_135719 Admin 
+
+Administrator 
+--{ RES }--
+V;200
+--{ CFG }--
+999;219;32;
+14;-1;5;-1;-1;-1;-1;-1;312;313;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;16;16;-1;104;-1;12;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;-1;
+998;0;
+998;3;DiTi 1000ul;DiTi 1000ul;DiTi 1000ul;
+998;1000-1;1000-2;1000-3;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;16;Tube Greinerconic 2mL 16 Pos;;;;;;;;;;;;;;;;
+998;Reactives;;;;;;;;;;;;;;;;
+998;1;Tube Eppendorf 6x 16 Pos;
+998;Proben;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;3;Trough 100ml;Trough 100ml;Trough 100ml;
+998;1-VEL-ElutionBuffer;5-VEW2-WashBuffer;4-VEW1 Wash Buffe;
+998;3;;Trough 100ml;;
+998;;Labware2;;
+998;0;
+998;8;Washstation 2Grid Cleaner short;Washstation 2Grid Waste;Washstation 2Grid Cleaner long;Trough 100ml;Trough 100ml;Trough 100ml;Washstation 2Grid DiTi Waste;;
+998;;;;3-VEB Binding Buffer;2-Vl Lysis Buffer;7-EtOH80p;;;
+998;0;
+998;3;96 Well DeepWell square;96 Well DeepWell square;96 Well DeepWell square;
+998;Plate lysis-1;Plate lysis-2;Plate lysis-3;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
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+998;0;
+998;0;
+998;0;
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+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
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+998;0;
+998;0;
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+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;0;
+998;2;
+998;4;0;System;
+998;4;1;Te-MagS portrait;
+998;1;
+998;37;Tube Eppendorf 48 Pos;
+998;1;
+998;15;
+996;0;0;
+--{ RPG }--
+Comment("Prefill 1 plates with LysisBufferReact for 96 samples.");
+Set_DITI_Counter2("DiTi 1000ul","1","1","1",0);
+Wash(15,23,1,23,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
+Group("Prefill plates with LysisBufferReact");
+UserPrompt("Put the plates for LysisBufferReact",1,-1);
+Group("Distribute: 100.0 �L of VL - Lysis Buffer ");
+Comment("Distribute: 100.0 �L of VL - Lysis Buffer (9984.0 �L total) from [grid:23 site:5 ['well 1 in labware 2-Vl Lysis Buffer: with 9984.0 uL of reagent VL - Lysis Buffer '] into Plate lysis-1[grid:25 site:1] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:");
+GetDITI2(15,"DiTi 1000ul",0,0,0,300);
+Aspirate(15,"MN VL","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08?0000000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080N000000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800l00000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000�0000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000�100000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000�30000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000p7000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000?00000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000N0000",0,0);
+Aspirate(15,"MN VL","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000l000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000000�00",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000�1",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000N",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000?0",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000p700",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000�3000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000�10000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000�000000",0,0);
+Aspirate(15,"MN VL","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000l0000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000N00000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000?000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800p70000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080�300000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08�1000000000000",0,0);
+GroupEnd();
+DropDITI(15,23,6,0.00,300,0);
+GroupEnd();

protocols/Prefill_plates_LysisBuffer/scripts/Prefill_LysisBuffer_1_plate.gwl

@@ -0,0 +1,34 @@
+B;Comment("Prefill 1 plates with LysisBufferReact for 96 samples.");
+B;Set_DITI_Counter2("DiTi 1000ul","1","1","1",0);
+B;Wash(15,23,1,23,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
+B;UserPrompt("Put the plates for LysisBufferReact",1,-1);
+B;Comment("Distribute: 100.0 �L of VL - Lysis Buffer (9984.0 �L total) from [grid:23 site:5 ['well 1 in labware 2-Vl Lysis Buffer: with 9984.0 uL of reagent VL - Lysis Buffer '] into Plate lysis-1[grid:25 site:1] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:");
+B;GetDITI2(15,"DiTi 1000ul",0,0,0,300);
+B;Aspirate(15,"MN VL","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08?0000000000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080N000000000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800l00000000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000�0000000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000�100000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000�30000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000p7000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000?00000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000N0000",0,0);
+B;Aspirate(15,"MN VL","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000l000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000000�00",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000�1",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000N",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000?0",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000p700",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000�3000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000�10000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000�000000",0,0);
+B;Aspirate(15,"MN VL","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000l0000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000N00000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000?000000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800p70000000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080�300000000000",0,0);
+B;Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08�1000000000000",0,0);
+B;DropDITI(15,23,6,0.00,300,0);

protocols/Prefill_plates_LysisBuffer/scripts/Prefill_LysisBuffer_1_plate.protocol.txt

@@ -0,0 +1,6 @@
+Prefill 1 plates with LysisBufferReact for 96 samples.
+
+Prefill plates with LysisBufferReact
+
+ Distribute: 100.0 �L of VL - Lysis Buffer 
+ Distribute: 100.0 �L of VL - Lysis Buffer (9984.0 �L total) from [grid:23 site:5 ['well 1 in labware 2-Vl Lysis Buffer: with 9984.0 uL of reagent VL - Lysis Buffer '] into Plate lysis-1[grid:25 site:1] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:

protocols/Prefill_plates_LysisBuffer/scripts/Prefill_LysisBuffer_1_plate.txt

@@ -0,0 +1,38 @@
+Comment("Prefill 1 plates with LysisBufferReact for 96 samples.");
+Set_DITI_Counter2("DiTi 1000ul","1","1","1",0);
+Wash(15,23,1,23,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
+Group("Prefill plates with LysisBufferReact");
+UserPrompt("Put the plates for LysisBufferReact",1,-1);
+Group("Distribute: 100.0 �L of VL - Lysis Buffer ");
+Comment("Distribute: 100.0 �L of VL - Lysis Buffer (9984.0 �L total) from [grid:23 site:5 ['well 1 in labware 2-Vl Lysis Buffer: with 9984.0 uL of reagent VL - Lysis Buffer '] into Plate lysis-1[grid:25 site:1] in order [1, 2, 3, 4, 9, 10, 11, 12, 17, 18, 19, 20, 25, 26, 27, 28, 33, 34, 35, 36, 41, 42, 43, 44, 49, 50, 51, 52, 57, 58, 59, 60, 65, 66, 67, 68, 73, 74, 75, 76, 81, 82, 83, 84, 89, 90, 91, 92, 93, 94, 95, 96, 85, 86, 87, 88, 77, 78, 79, 80, 69, 70, 71, 72, 61, 62, 63, 64, 53, 54, 55, 56, 45, 46, 47, 48, 37, 38, 39, 40, 29, 30, 31, 32, 21, 22, 23, 24, 13, 14, 15, 16, 5, 6, 7, 8]:");
+GetDITI2(15,"DiTi 1000ul",0,0,0,300);
+Aspirate(15,"MN VL","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08?0000000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080N000000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800l00000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000�0000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000�100000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000�30000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000p7000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000?00000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000N0000",0,0);
+Aspirate(15,"MN VL","900.00","900.00","900.00","900.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000l000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000000�00",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000�1",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000000N",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000000?0",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000000p700",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000000�3000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000000�10000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000000�000000",0,0);
+Aspirate(15,"MN VL","600.00","600.00","600.00","600.00",0,0,0,0,0,0,0,0,23,4,1,"0108l0",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08000000l0000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800000N00000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080000?000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C0800p70000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C080�300000000000",0,0);
+Dispense(15,"MN VL","100.00","100.00","100.00","100.00",0,0,0,0,0,0,0,0,25,0,1,"0C08�1000000000000",0,0);
+GroupEnd();
+DropDITI(15,23,6,0.00,300,0);
+GroupEnd();