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review Tutorial_HL
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#9 #37 #45
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qPCR4vir committed Aug 20, 2019
1 parent 7753a84 commit 89ba57e
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Showing 13 changed files with 41 additions and 45 deletions.
6 changes: 3 additions & 3 deletions protocols/tests/Tutorial_HLevel_46s.esc
Original file line number Diff line number Diff line change
Expand Up @@ -141,7 +141,7 @@ V;200
996;0;0;
--{ RPG }--
Comment("Dilute 1:10 mix1 in 46 wells.");
UserPrompt("Check Diluent in ['well 1 in labware BufferCub: with 4305.0 uL of reagent Diluent'] Check mix1 in ['well 1 in labware mixes: with 478.0 uL of reagent mix1'] ",1,-1);
UserPrompt("Check diluent in ['well 1 in labware BufferCub: with 4305.0 uL of reagent diluent'] Check mix1 in ['well 1 in labware mixes: with 478.0 uL of reagent mix1'] ",1,-1);
Wash(255,19,1,19,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
Group("Fill dilutions");
UserPrompt("Put the plate for dilutions in grid:55, site:3",1,-1);
Expand All @@ -164,8 +164,8 @@ Dispense(255,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","10.00
Dispense(63,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0",0,0,0,0,55,2,1,"0C0800000�?0000000",0,0);
GroupEnd();
DropDITI(255,19,6,0.00,300,0);
Group("Distribute: 90.0 �L of Diluent");
Comment("Distribute: 90.0 �L of Diluent (4305.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 4305.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:");
Group("Distribute: 90.0 �L of diluent");
Comment("Distribute: 90.0 �L of diluent (4305.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 4305.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:");
GetDITI2(255,"DiTi 1000ul",0,0,0,300);
Aspirate(255,"Water free","540.00","540.00","540.00","540.00","540.00","540.00","450.00","450.00",0,0,0,0,53,0,1,"0108�1",0,0);
Dispense(255,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","90.00","90.00",0,0,0,0,55,2,1,"0C08�1000000000000",0,0);
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4 changes: 2 additions & 2 deletions protocols/tests/Tutorial_HLevel_46s.gwl
Original file line number Diff line number Diff line change
@@ -1,5 +1,5 @@
B;Comment("Dilute 1:10 mix1 in 46 wells.");
B;UserPrompt("Check Diluent in ['well 1 in labware BufferCub: with 4305.0 uL of reagent Diluent'] Check mix1 in ['well 1 in labware mixes: with 478.0 uL of reagent mix1'] ",1,-1);
B;UserPrompt("Check diluent in ['well 1 in labware BufferCub: with 4305.0 uL of reagent diluent'] Check mix1 in ['well 1 in labware mixes: with 478.0 uL of reagent mix1'] ",1,-1);
B;Wash(255,19,1,19,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
B;UserPrompt("Put the plate for dilutions in grid:55, site:3",1,-1);
B;Comment("Distribute: 10.0 �L of mix1 (478.4 �L total) from [grid:12 site:1 ['well 1 in labware mixes: with 478.0 uL of reagent mix1'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:");
Expand All @@ -19,7 +19,7 @@ B;Dispense(255,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","10.
B;Dispense(255,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","10.00","10.00",0,0,0,0,55,2,1,"0C080000�O00000000",0,0);
B;Dispense(63,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0",0,0,0,0,55,2,1,"0C0800000�?0000000",0,0);
B;DropDITI(255,19,6,0.00,300,0);
B;Comment("Distribute: 90.0 �L of Diluent (4305.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 4305.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:");
B;Comment("Distribute: 90.0 �L of diluent (4305.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 4305.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:");
B;GetDITI2(255,"DiTi 1000ul",0,0,0,300);
B;Aspirate(255,"Water free","540.00","540.00","540.00","540.00","540.00","540.00","450.00","450.00",0,0,0,0,53,0,1,"0108�1",0,0);
B;Dispense(255,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","90.00","90.00",0,0,0,0,55,2,1,"0C08�1000000000000",0,0);
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4 changes: 2 additions & 2 deletions protocols/tests/Tutorial_HLevel_46s.protocol.txt
Original file line number Diff line number Diff line change
Expand Up @@ -5,5 +5,5 @@ Fill dilutions
Distribute: 10.0 �L of mix1
Distribute: 10.0 �L of mix1 (478.4 �L total) from [grid:12 site:1 ['well 1 in labware mixes: with 478.0 uL of reagent mix1'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:

Distribute: 90.0 �L of Diluent
Distribute: 90.0 �L of Diluent (4305.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 4305.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:
Distribute: 90.0 �L of diluent
Distribute: 90.0 �L of diluent (4305.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 4305.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:
6 changes: 3 additions & 3 deletions protocols/tests/Tutorial_HLevel_46s.txt
Original file line number Diff line number Diff line change
@@ -1,5 +1,5 @@
Comment("Dilute 1:10 mix1 in 46 wells.");
UserPrompt("Check Diluent in ['well 1 in labware BufferCub: with 4305.0 uL of reagent Diluent'] Check mix1 in ['well 1 in labware mixes: with 478.0 uL of reagent mix1'] ",1,-1);
UserPrompt("Check diluent in ['well 1 in labware BufferCub: with 4305.0 uL of reagent diluent'] Check mix1 in ['well 1 in labware mixes: with 478.0 uL of reagent mix1'] ",1,-1);
Wash(255,19,1,19,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
Group("Fill dilutions");
UserPrompt("Put the plate for dilutions in grid:55, site:3",1,-1);
Expand All @@ -22,8 +22,8 @@ Dispense(255,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","10.00
Dispense(63,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0",0,0,0,0,55,2,1,"0C0800000�?0000000",0,0);
GroupEnd();
DropDITI(255,19,6,0.00,300,0);
Group("Distribute: 90.0 �L of Diluent");
Comment("Distribute: 90.0 �L of Diluent (4305.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 4305.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:");
Group("Distribute: 90.0 �L of diluent");
Comment("Distribute: 90.0 �L of diluent (4305.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 4305.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]:");
GetDITI2(255,"DiTi 1000ul",0,0,0,300);
Aspirate(255,"Water free","540.00","540.00","540.00","540.00","540.00","540.00","450.00","450.00",0,0,0,0,53,0,1,"0108�1",0,0);
Dispense(255,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","90.00","90.00",0,0,0,0,55,2,1,"0C08�1000000000000",0,0);
Expand Down
6 changes: 3 additions & 3 deletions protocols/tests/Tutorial_HLevel_6s.esc
Original file line number Diff line number Diff line change
Expand Up @@ -141,7 +141,7 @@ V;200
996;0;0;
--{ RPG }--
Comment("Dilute 1:10 mix1 in 6 wells.");
UserPrompt("Check Diluent in ['well 1 in labware BufferCub: with 561.0 uL of reagent Diluent'] Check mix1 in ['well 1 in labware mixes: with 62.0 uL of reagent mix1'] ",1,-1);
UserPrompt("Check diluent in ['well 1 in labware BufferCub: with 561.0 uL of reagent diluent'] Check mix1 in ['well 1 in labware mixes: with 62.0 uL of reagent mix1'] ",1,-1);
Wash(255,19,1,19,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
Group("Fill dilutions");
UserPrompt("Put the plate for dilutions in grid:55, site:3",1,-1);
Expand All @@ -157,8 +157,8 @@ Aspirate(32,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0"
Dispense(63,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0",0,0,0,0,55,2,1,"0C08o0000000000000",0,0);
GroupEnd();
DropDITI(63,19,6,0.00,300,0);
Group("Distribute: 90.0 �L of Diluent");
Comment("Distribute: 90.0 �L of Diluent (561.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 561.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:");
Group("Distribute: 90.0 �L of diluent");
Comment("Distribute: 90.0 �L of diluent (561.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 561.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:");
GetDITI2(63,"DiTi 1000ul",0,0,0,300);
Aspirate(63,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","0","0",0,0,0,0,53,0,1,"0108�0",0,0);
Dispense(63,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","0","0",0,0,0,0,55,2,1,"0C08o0000000000000",0,0);
Expand Down
4 changes: 2 additions & 2 deletions protocols/tests/Tutorial_HLevel_6s.gwl
Original file line number Diff line number Diff line change
@@ -1,5 +1,5 @@
B;Comment("Dilute 1:10 mix1 in 6 wells.");
B;UserPrompt("Check Diluent in ['well 1 in labware BufferCub: with 561.0 uL of reagent Diluent'] Check mix1 in ['well 1 in labware mixes: with 62.0 uL of reagent mix1'] ",1,-1);
B;UserPrompt("Check diluent in ['well 1 in labware BufferCub: with 561.0 uL of reagent diluent'] Check mix1 in ['well 1 in labware mixes: with 62.0 uL of reagent mix1'] ",1,-1);
B;Wash(255,19,1,19,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
B;UserPrompt("Put the plate for dilutions in grid:55, site:3",1,-1);
B;Comment("Distribute: 10.0 �L of mix1 (62.4 �L total) from [grid:12 site:1 ['well 1 in labware mixes: with 62.0 uL of reagent mix1'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:");
Expand All @@ -12,7 +12,7 @@ B;Aspirate(16,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","
B;Aspirate(32,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0",0,0,0,0,12,0,1,"0110100",0,0);
B;Dispense(63,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0",0,0,0,0,55,2,1,"0C08o0000000000000",0,0);
B;DropDITI(63,19,6,0.00,300,0);
B;Comment("Distribute: 90.0 �L of Diluent (561.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 561.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:");
B;Comment("Distribute: 90.0 �L of diluent (561.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 561.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:");
B;GetDITI2(63,"DiTi 1000ul",0,0,0,300);
B;Aspirate(63,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","0","0",0,0,0,0,53,0,1,"0108�0",0,0);
B;Dispense(63,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","0","0",0,0,0,0,55,2,1,"0C08o0000000000000",0,0);
Expand Down
4 changes: 2 additions & 2 deletions protocols/tests/Tutorial_HLevel_6s.protocol.txt
Original file line number Diff line number Diff line change
Expand Up @@ -5,5 +5,5 @@ Fill dilutions
Distribute: 10.0 �L of mix1
Distribute: 10.0 �L of mix1 (62.4 �L total) from [grid:12 site:1 ['well 1 in labware mixes: with 62.0 uL of reagent mix1'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:

Distribute: 90.0 �L of Diluent
Distribute: 90.0 �L of Diluent (561.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 561.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:
Distribute: 90.0 �L of diluent
Distribute: 90.0 �L of diluent (561.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 561.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:
6 changes: 3 additions & 3 deletions protocols/tests/Tutorial_HLevel_6s.txt
Original file line number Diff line number Diff line change
@@ -1,5 +1,5 @@
Comment("Dilute 1:10 mix1 in 6 wells.");
UserPrompt("Check Diluent in ['well 1 in labware BufferCub: with 561.0 uL of reagent Diluent'] Check mix1 in ['well 1 in labware mixes: with 62.0 uL of reagent mix1'] ",1,-1);
UserPrompt("Check diluent in ['well 1 in labware BufferCub: with 561.0 uL of reagent diluent'] Check mix1 in ['well 1 in labware mixes: with 62.0 uL of reagent mix1'] ",1,-1);
Wash(255,19,1,19,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
Group("Fill dilutions");
UserPrompt("Put the plate for dilutions in grid:55, site:3",1,-1);
Expand All @@ -15,8 +15,8 @@ Aspirate(32,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0"
Dispense(63,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","0","0",0,0,0,0,55,2,1,"0C08o0000000000000",0,0);
GroupEnd();
DropDITI(63,19,6,0.00,300,0);
Group("Distribute: 90.0 �L of Diluent");
Comment("Distribute: 90.0 �L of Diluent (561.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 561.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:");
Group("Distribute: 90.0 �L of diluent");
Comment("Distribute: 90.0 �L of diluent (561.6 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 561.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6]:");
GetDITI2(63,"DiTi 1000ul",0,0,0,300);
Aspirate(63,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","0","0",0,0,0,0,53,0,1,"0108�0",0,0);
Dispense(63,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","0","0",0,0,0,0,55,2,1,"0C08o0000000000000",0,0);
Expand Down
6 changes: 3 additions & 3 deletions protocols/tutorial_HL/scripts/dilutions_HL_42s.esc
Original file line number Diff line number Diff line change
Expand Up @@ -141,7 +141,7 @@ V;200
996;0;0;
--{ RPG }--
Comment("Dilute 1:10 mix1 in 42 wells.");
UserPrompt("Check Diluent in ['well 1 in labware BufferCub: with 3931.0 uL of reagent Diluent'] Check mix1 in ['well 1 in labware mixes: with 436.0 uL of reagent mix1'] ",1,-1);
UserPrompt("Check diluent in ['well 1 in labware BufferCub: with 3931.0 uL of reagent diluent'] Check mix1 in ['well 1 in labware mixes: with 436.0 uL of reagent mix1'] ",1,-1);
Wash(255,19,1,19,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
Group("Fill dilutions");
UserPrompt("Put the plate for dilutions in grid:55, site:3",1,-1);
Expand All @@ -164,8 +164,8 @@ Dispense(255,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","10.00
Dispense(3,"Water free","10.00","10.00","0","0","0","0","0","0",0,0,0,0,55,2,1,"0C0800000�00000000",0,0);
GroupEnd();
DropDITI(255,19,6,0.00,300,0);
Group("Distribute: 90.0 �L of Diluent");
Comment("Distribute: 90.0 �L of Diluent (3931.2 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 3931.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42]:");
Group("Distribute: 90.0 �L of diluent");
Comment("Distribute: 90.0 �L of diluent (3931.2 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 3931.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42]:");
GetDITI2(255,"DiTi 1000ul",0,0,0,300);
Aspirate(255,"Water free","540.00","540.00","450.00","450.00","450.00","450.00","450.00","450.00",0,0,0,0,53,0,1,"0108�1",0,0);
Dispense(255,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","90.00","90.00",0,0,0,0,55,2,1,"0C08�1000000000000",0,0);
Expand Down
4 changes: 2 additions & 2 deletions protocols/tutorial_HL/scripts/dilutions_HL_42s.gwl
Original file line number Diff line number Diff line change
@@ -1,5 +1,5 @@
B;Comment("Dilute 1:10 mix1 in 42 wells.");
B;UserPrompt("Check Diluent in ['well 1 in labware BufferCub: with 3931.0 uL of reagent Diluent'] Check mix1 in ['well 1 in labware mixes: with 436.0 uL of reagent mix1'] ",1,-1);
B;UserPrompt("Check diluent in ['well 1 in labware BufferCub: with 3931.0 uL of reagent diluent'] Check mix1 in ['well 1 in labware mixes: with 436.0 uL of reagent mix1'] ",1,-1);
B;Wash(255,19,1,19,0,"5",50,"10",50,0.00,50,100,1,0,0,0);
B;UserPrompt("Put the plate for dilutions in grid:55, site:3",1,-1);
B;Comment("Distribute: 10.0 �L of mix1 (436.8 �L total) from [grid:12 site:1 ['well 1 in labware mixes: with 436.0 uL of reagent mix1'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42]:");
Expand All @@ -19,7 +19,7 @@ B;Dispense(255,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","10.
B;Dispense(255,"Water free","10.00","10.00","10.00","10.00","10.00","10.00","10.00","10.00",0,0,0,0,55,2,1,"0C080000�O00000000",0,0);
B;Dispense(3,"Water free","10.00","10.00","0","0","0","0","0","0",0,0,0,0,55,2,1,"0C0800000�00000000",0,0);
B;DropDITI(255,19,6,0.00,300,0);
B;Comment("Distribute: 90.0 �L of Diluent (3931.2 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 3931.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42]:");
B;Comment("Distribute: 90.0 �L of diluent (3931.2 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 3931.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42]:");
B;GetDITI2(255,"DiTi 1000ul",0,0,0,300);
B;Aspirate(255,"Water free","540.00","540.00","450.00","450.00","450.00","450.00","450.00","450.00",0,0,0,0,53,0,1,"0108�1",0,0);
B;Dispense(255,"Water free","90.00","90.00","90.00","90.00","90.00","90.00","90.00","90.00",0,0,0,0,55,2,1,"0C08�1000000000000",0,0);
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4 changes: 2 additions & 2 deletions protocols/tutorial_HL/scripts/dilutions_HL_42s.protocol.txt
Original file line number Diff line number Diff line change
Expand Up @@ -5,5 +5,5 @@ Fill dilutions
Distribute: 10.0 �L of mix1
Distribute: 10.0 �L of mix1 (436.8 �L total) from [grid:12 site:1 ['well 1 in labware mixes: with 436.0 uL of reagent mix1'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42]:

Distribute: 90.0 �L of Diluent
Distribute: 90.0 �L of Diluent (3931.2 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 3931.0 uL of reagent Diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42]:
Distribute: 90.0 �L of diluent
Distribute: 90.0 �L of diluent (3931.2 �L total) from [grid:53 site:1 ['well 1 in labware BufferCub: with 3931.0 uL of reagent diluent'] into plate[grid:55 site:3] in order [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42]:
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