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Add/fix new test, with RoMA
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#29, #9, #19, 34
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qPCR4vir committed Aug 18, 2019
1 parent dc711a9 commit faa54a1
Showing 1 changed file with 33 additions and 34 deletions.
67 changes: 33 additions & 34 deletions protocols/Prefill_plate_in_Evo200/Prefill_plate_in_Evo200.py
Original file line number Diff line number Diff line change
Expand Up @@ -57,11 +57,10 @@ def Run(self):
self.comment('Prefill a plate with some dilutions of two master mix and Buffer Reagent for {:d} samples.'
.format(num_of_samples))


BufCuvette = wt.get_labware("BufferCub", labware.Trough_100ml) # Get Labwares from the work table
master_mixes_ = wt.get_labware("mixes", labware.Eppendorfrack)

maxTips = min (self.n_tips, num_of_samples)
maxTips = min(self.n_tips, num_of_samples)

buf_per_sample =0
well_v = 100
Expand All @@ -86,17 +85,17 @@ def Run(self):
# Define the reactives in each labware (Cuvette, eppys, etc.)

buffer_reag = Reagent("Buffer ",
BufCuvette,
volpersample = buf_per_sample,
# defLiqClass = 'MN VL',
# num_of_samples= num_of_samples
)

mix1 =Reagent("mix1",
master_mixes_,
volpersample = dil_mix1_10,
# defLiqClass = 'MN VL'
)
BufCuvette,
volpersample = buf_per_sample,
# defLiqClass = 'MN VL',
# num_of_samples= num_of_samples
)

mix1 = Reagent("mix1",
master_mixes_,
volpersample = dil_mix1_10,
# defLiqClass = 'MN VL'
)

mix2 = Reagent("mix2",
master_mixes_,
Expand All @@ -110,40 +109,40 @@ def Run(self):

instructions.wash_tips(wasteVol=5, FastWash=True).exec()

Plat1 = wt.get_labware("plate1", labware.MP96MachereyNagel)
Plat2 = wt.get_labware("plate2", labware.MP96well)
plate_1 = wt.get_labware("plate1", labware.MP96MachereyNagel)
plate_2 = wt.get_labware("plate2", labware.MP96well)

# Define place for intermediate reactions
Reagent.use_minimal_number_of_aliquots = False
mix1_10 = Reagent(f"mix1, diluted 1:10",
Plat1,
initial_vol = 0.0,
replicas = num_of_samples,
excess = 0)
plate_1,
initial_vol = 0.0,
replicas = num_of_samples,
excess = 0)

mix2_10 = Reagent(f"mix2, diluted 1:10",
Plat1,
initial_vol = 0.0,
replicas = num_of_samples,
excess = 0)
plate_1,
initial_vol = 0.0,
replicas = num_of_samples,
excess = 0)

mix1_100 = Reagent(f"mix1, diluted 1:100",
Plat2,
initial_vol=0.0,
replicas=num_of_samples,
excess=0)
plate_2,
initial_vol=0.0,
replicas=num_of_samples,
excess=0)

mix2_100 = Reagent(f"mix2, diluted 1:100",
Plat2,
initial_vol=0.0,
replicas=num_of_samples,
excess=0)
plate_2,
initial_vol=0.0,
replicas=num_of_samples,
excess=0)

loc = Plat2.location # just showing how to move the plate from one site to the next in the carrier
loc = plate_2.location # just showing how to move the plate from one site to the next in the carrier
loc.site -= 1
carrier = labware.Carrier(labware.Carrier.Type.by_name["MP 3Pos"], loc.grid, label ="MP 3Pos")
carrier = labware.Carrier(self.get_carrier_type("MP 3Pos"), loc.grid, label ="MP 3Pos")
loc.carrier = carrier
instructions.transfer_rack(Plat2, loc ).exec() # just showing how RoMa works.
instructions.transfer_rack(plate_2, loc).exec() # just showing how RoMa works.

with group("Fill plate with mixes "):

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