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Improve DE tutorial #615

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grst opened this issue May 27, 2024 · 1 comment
Open

Improve DE tutorial #615

grst opened this issue May 27, 2024 · 1 comment
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enhancement New feature or request

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@grst
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grst commented May 27, 2024

Description of feature

In addition to the basic DE tutorial that shows the basic functionality of DE methods, we should have an advanced tutorial that shows how to build more complex contrasts, including interaction terms based on a real-world multi-condition dataset/atlas.

Some work has been d started here, but didn't progress far: https://github.com/scverse/multi-condition-comparisions/blob/main/docs/notebooks/breast_cancer.ipynb

@grst grst added the enhancement New feature or request label May 27, 2024
@Zethson Zethson self-assigned this Jun 11, 2024
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Zethson commented Jun 12, 2024

Added a rough draft https://github.com/scverse/pertpy-tutorials/blob/main/differential_gene_expression.ipynb

TODOs

  • Maybe make the patients as independent covariates work (leads to matrix not being full rank)
  • Better explain what the DE results mean and how they can be used now (maybe a dirty decoupler/GSEA/something analysis
  • Better explain the models
  • Better explain how design matrices and contrasts work (just 1-2 sentences each)
  • Incorporate better visualizations as they become available in pertpy
  • Show how to use scanpy plotting functions with the DE results

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