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README.md v2024-04-16, includes notes on release v2.2.1
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vinuesa committed Apr 16, 2024
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Expand Up @@ -14,16 +14,17 @@ Starting with **release 2.0.0** (2022-11-20), a **concatenation-independent spec

[**Release 2.2.0** (v2.2.0, 2024-04-14)](https://github.com/vinuesa/get_phylomarkers/releases/tag/2.2.0) contains new features, significant code improvements, binary updates, and some bug fixes. Among the new features, we would like to highlight the following ones:

-significant extension of run mode 2 (<code>run_get_phylomarkers_pipeline.sh -R 2</code>) for **population genetics** (multiple sequences from the same species):
- significant extension of run mode 2 (<code>run_get_phylomarkers_pipeline.sh -R 2</code>) for **population genetics** (multiple sequences from the same species)

- <code>run_get_phylomarkers_pipeline.sh</code> now also calls the C binary [**WEIGHTED-ASTRAL**](https://github.com/chaoszhang/ASTER) to estimate a species tree using as input the filtered gene trees estimated by [iqtree2](http://www.iqtree.org/) or [FastTree](http://www.microbesonline.org/fasttree/) from the core-genome clusters computed by [get_homologues](https://github.com/eead-csic-compbio/get_homologues).

- The main script <code>run_get_phylomarkers_pipeline.sh</code> added complex protein mixture models for concatenated protein alignments, prints script invocation arguments to STDOU at the beginning of the run, and prints a summary results of the different filtering steps before exiting. Read the [**Release v2.2.0**)](https://github.com/vinuesa/get_phylomarkers/releases/tag/2.2.0) notes for further details.

- Users of this release interested in run mode 2 (-R 2) to compute a population tree, will need to install [snp-sites](https://github.com/sanger-pathogens/snp-sites), as explained in the current [INSTALL.md notes](https://github.com/vinuesa/get_phylomarkers/blob/master/INSTALL.md).

- Linux users that cloned the the [GET_PHYLOMARKERS GitHub repository](https://github.com/vinuesa/get_phylomarkers) on **2024-04-15**, or later, will not have to install [snp-sites](https://github.com/sanger-pathogens/snp-sites) manually, because a static binary is provided (bin/linux/snp-sites-static), which is called by <code>run_get_phylomarkers_pipeline.sh v2.8.1.0_2024-04-15</code>. Thanks to Alfredo Hernández @ccg_unam for compiling snp-sites-static.
[**Release 2.2.1** (2024-04-16)](https://github.com/vinuesa/get_phylomarkers/releases/tag/2.2.1) and Linux users that cloned the the [GET_PHYLOMARKERS GitHub repository](https://github.com/vinuesa/get_phylomarkers) on **2024-04-15**, or later, will not have to install [snp-sites](https://github.com/sanger-pathogens/snp-sites) manually, because a static binary is provided (bin/linux/snp-sites-static), which is called by <code>run_get_phylomarkers_pipeline.sh >= v2.8.1.0_2024-04-15</code>. Thanks to Alfredo Hernández @ccg_unam for compiling snp-sites-static.

- [**Release 2.2.0** (2024-04-14)](https://github.com/vinuesa/get_phylomarkers/releases/tag/2.2.0) was used to build the *latest* **Docker image** ready to pull from [**Docker Hub**](https://hub.docker.com/r/vinuesa/get_phylomarkers). On Dockerhub, you will find detailed instructions on installing and configuring the Docker client on your machine, pulling the latest image, and running the containerized instance of the GET_PHYLOMARKERS pipeline.
- [**Release 2.2.1** (2024-04-16)](https://github.com/vinuesa/get_phylomarkers/releases/tag/2.2.1) was used to build the *latest* **Docker image v20240415** ready to pull from [**Docker Hub**](https://hub.docker.com/r/vinuesa/get_phylomarkers). On Dockerhub, you will find detailed instructions on installing and configuring the Docker client on your machine, pulling the latest image, and running the containerized instance of the GET_PHYLOMARKERS pipeline.


**GET_PHYLOMARKERS** can also estimate **ML and parsimony trees from the pan-genome matrix**, including unsupervised learning methods to determine the optimal number of clusters from the pan-genome and average genomic distance matrices. A detailed [**manual**](https://vinuesa.github.io/get_phylomarkers/#get_phylomarkers-manual) and step-by-step [**tutorials**](https://vinuesa.github.io/get_phylomarkers/#get_phylomarkers-tutorial) document the software and help the user to get up and running quickly. For convenience, [**html**](https://vinuesa.github.io/get_phylomarkers/) and [**markdown**](https://github.com/vinuesa/get_phylomarkers/blob/master/docs/GET_PHYLOMARKERS_manual.md) versions of the documentation material are available.
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