Amide ¹⁵N relaxation experiments

NMR pulse sequences of amide pseudo-3D ¹⁵N R₁, R_1ρ, and {¹H}-¹⁵N steady-state heteronuclear NOE relaxation experiments, shapes, and parameter sets for Bruker spectrometers.

Requirements

• Topspin 3.6 or above
• Avance III console or above

Tested with Topspin 3.6 and Avance III console.

Details

• Temperature compensation during d1
• ¹H amide-selective 180^o pulses for cross-correlated relaxation suppression
• Adiabatic half passage pulses for accurate magnetization alignment in R_1ρ experiments

Setup

For detailed information, please read the protocol (REF TBD). You will also find setup notes in the headers of the pulse sequence files as well as the shapes and the parameters folders.

References

• X Xiang, AL Hansen, L Bruschweiler-Li, R Brüschweiler, M Xie. Under review. doi: [] ()
• N-A Lakomek, J Ying, A Bax. J Biomol NMR (2012) 53:209-221. doi: 10.1007/s10858-012-9626-5
• M Gairi, A Dyachenko, MT Gonzalez, M Feliz, M Pons, E Giralt. J Biomol NMR (2015) 62:209-220. doi: 10.1007/s10858-015-9937-4
• FA Mulder, RA de Graaf, R Kaptein, R Boelens. J Magn Reson (1998) 131.2:351-357. doi: 10.1006/jmre.1998.1380
• AG Palmer III, CD Kroenke, JP Loria. Methods Enzymol (2001) 339:204-38. doi: 10.1016/S0076-6879(01)39315-1

Contact

Alexandar L Hansen,