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BIDCatUCSF/fiji-xytbleachcorrect
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// Name: // wideguy_xyt_bleachcorrect_Imaris_v2.txt // // Description: // fiji macro for performing xyt Bleach Correction using the histogram // matching method: // // https://imagej.net/Bleach_Correction // // and file conversion to be opened in Imaris. Inspired by BIDC's // wideguy microscope, Ed Roberts and Kelly Kersten // // Usage: // 0) Create a folder for input images and one for resultant output images // 1) Open your image stack in FIJI // 2) In FIJI: // Plugins -> Macros -> Run... // and select the text file named wideguy_xyt_bleachcorrect_Imaris.txt // 3) Choose the input folder // 4) Choose the output folder // 5) From the popup window select which channels you wish to perform the bleach correction // 5) Grab coffee // // Output: // .ics and .ids file for each image stack // // to open the output in Imaris, drag the .ics file into Imaris' Surpass // // Author: // Adam Fries 2018.02.21 // // Details: // Intended only for use of 2-channel time-series from uManager output files // Does not work for z-stacks...yet // // Dependencies: // FIJI BleachCorrection plugin // FIJI BioFormats
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XYT Bleach Correction Imaris Output
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