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Merge branch 'master' of https://github.com/COMBINE-lab/minnow
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hiraksarkar committed Feb 7, 2019
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[docker](docker pull hrksrkr/minnow)
[tutorial](https://combine-lab.github.io/alevin-tutorial/2019/running-minnow/)
[![Build Status](https://travis-ci.org/COMBINE-lab/salmon.svg?branch=master)](https://travis-ci.org/COMBINE-lab/salmon)

[![Build Status](https://travis-ci.org/COMBINE-lab/minnow.svg?branch=master)](https://travis-ci.org/COMBINE-lab/minnow)
# Minnow ( read level simulator for dscRNA-seq data)

Most analysis pipelines validate their results using known marker genes (which are not widely available for all types of analysis) and by using simulated data from gene-count-level simulators. Typically, the impact of using different read-alignment or UMI deduplication methods has not been widely explored. Assessments based on simulation tend to start at the level of assuming a simulated count matrix, ignoring the effect that different approaches for resolving UMI counts from the raw read data may produce. Here, we present minnow, a comprehensive sequence-level droplet-based single-cell RNA-seq (dscRNA-seq) experiment simulation framework. Minnow accounts for important sequence-level characteristics of experimental scRNA-seq datasets and models effects such as PCR amplification, CB (cellular barcodes) and UMI (Unique Molecule Identifiers) selection, and sequence fragmentation and sequencing.
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<img src="data/minnow_main_figure.001.jpeg">
</p>

## Pre-build images

[docker](`docker pull hrksrkr/minnow`)

[tutorial](https://combine-lab.github.io/alevin-tutorial/2019/running-minnow/)


## Installation
Minnow is written in C++14 and tested in a ubuntu server, please let us know if you have difficulty compiling it in your own machine.
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