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Files and scripts to generate figures for the manuscript titled "Skin metatranscriptomics reveals landscape of variation in microbial activity and gene expression across the human body".

To run the scripts, clone this repository, download the large datasets from figshare and specify working directory to the scripts/ subfolder or open the project using the .Rproj file

Directory structure

  • scripts: Scripts used to generate all figures in the manuscript
  • data: Script outputs and intermediate data files
  • plots: Vectorized pdfs of figure panels
  • metadata: Metadata and annotations used as inputs into scripts

Raw data availability

Raw sequencing data has been uploaded to the European Nucleotide Archive (ENA), under project accession number PRJEB82796.
Large datasets and annotations have been uploaded to figshare here.

Contact

Please direct any questions or feedback to Minghao Chia and Niranjan Nagarajan.

Navigating the figures, scripts and plots

The metadata (skin sites and read counts) for the full cohort is here.
Please refer to repository map for the relationship between figure panels, scripts and plots in this repository.

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